TABLE 3.
Primers used for the construction and complementation of S. Enteritidis deletion mutants
| Primer | Sequence (5′ to 3′) (restriction enzyme)a |
|---|---|
| hiuH-F1 | CTCTAGAGCCGTCAGGCAAATAA (XbaI) |
| hiuH-R1 | AGGCTCTAAAGCTTCACTCCTTTACGGTAT |
| hiuH-F2 | GGAGTGAAGCTTTAGAGCCTATCCCATTAG |
| hiuH-R2 | GCGAGCTCAAGCGGGATAACCACC (SacI) |
| proX-F1 | CTCTAGAAGGTGCCTGCCGACTT (XbaI) |
| proX-R1 | AAAAACGATCCGTTGTTCCTTTAATTATGG |
| proX-F2 | AGGAACAACGGATCGTTTTTTATGCCGGAT |
| proX-R2 | GCGAGCTCTGCTAAGCGACTGACTGC (SacI) |
a
The 20-bp overlap sequences that were engineered into primers for amplification of the fragments of homologous arms are in bold, and restriction sites are underlined.