TABLE 4.
Strains and plasmids used for the construction and complementation of S. Enteritidis deletion mutants
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| S. Enteritidis ATCC 13076 | Wild-type strain | American Type Culture Collection |
| ΔhiuH | hiuH deletion mutant of S. Enteritidis ATCC 13076 | This study |
| ΔhiuH-C | Complementary strain for hiuH deletion mutant | This study |
| ΔproX | proX deletion mutant of S. Enteritidis ATCC 13076 | This study |
| ΔproX-C | Complementary strain for proX deletion mutant | This study |
| E. coli DH5α | Host for cloning | Laboratory stock |
| E. coli SM10(λpir) | thi thr-1 leu6 proA2 his-4 arg E2 lacY1 galK2 ara14 xyl5 supE44 λpir | Laboratory stock |
| pMD19-T | Cloning vector, Ampr | TaKaRa, Japan |
| pRE112 | pGP704 suicide plasmid, pir dependent, oriT oriV sacB, Cmr | Laboratory stock |
| pRE112-ΔhiuH | pRE112 containing a 686-bp hiuH deletion PCR product | This study |
| pRE112-ΔhiuH-C | pRE112 containing a wild-type copy of hiuH and its two flanking sequences; used to complement strain ΔhiuH | This study |
| pRE112-ΔproX | pRE112 containing a 905-bp proX deletion PCR product | This study |
| pRE112-ΔproX-C | pRE112 containing a wild-type copy of proX and its two flanking sequences; used to complement strain ΔproX | This study |