. 2019 Sep 17;85(19):e00800-19. doi: 10.1128/AEM.00800-19

TABLE 2.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristic(s)^a	Source or reference
Strain
E. coli VE7108	Containing the repA plasmid gene (not thermosensitive); Km^r	54
L. gasseri DSM 14869	Parent strain	BifodanA/S, Denmark
14869-ΔN506_0400	14869 lacking the gene N506_0400	This study
14869-ΔN506_1778	14869 lacking the gene N506_1788	This study
14869-ΔN506_1709	14869 lacking the gene N506_1709	This study
14869-ΔN506_0400/pNZe-N506_0400	14869-ΔN506_0400 harboring plasmid pNZe-N506_0400	This study
14869-ΔN506_1778/pNZe-N506_1778	14869-ΔN506_1778 harboring pNZe-N506_1778	This study
14869-ΔN506_1709/pNZ8048-N506_1709	14869-ΔN506_1709 harboring pNZ8048-N506_1709	This study
Lactococcus lactis NZ9000	MG1363 pepN::nisRK	64
L. lactis NZ9000/pNZ8048	L. lactis NZ9000 harboring empty vector pNZ8048	This study
L. lactis NZ9000/pNZ8048-N506_1709Re	L. lactis NZ9000 harboring pNZ8048-N506_1709Re	This study
Plasmid
pINTZrec	Replicable plasmid-mediating homologous recombination; Cm^r	57
pINTZrec-N506_0400	pINTZrec derivative containing homologous regions up- and downstream of N506_0400; Cm^r	This study
pINTZrec-N506_1778	pINTZrec derivative containing homologous regions up- and downstream of N506_1778; Cm^r	This study
pINTZrec-N506_1709	pINTZrec derivative containing homologous regions up- and downstream of N506_1709; Cm^r	This study
pNZ8048	E. coli-lactic acid bacterium shuttle cloning vector; Cm^r	65
pNZe-Rec	pNZ8048 derivative; Em^r	Laboratory stock
pNZe-N506_0400	pNZe-Rec derivative containing N506_0400; Em^r	This study
pNZe-N506_1778	pNZe-N506_0400 derivative containing N506_1778; Em^r	This study
pNZ8048-N506_1709	pNZ8048 derivative containing N506_1709 and its promoter; Cm^r	This study
pNZ8048- N506_1709Re	pNZ8048 derivative containing N506_1709; Cm^r	This study

Km^r, kanamycin resistance; Cm^r, chloramphenicol resistance; Em^r, erythromycin resistance.