TABLE 2.
Description of RBS selected in this studya
| Primer | Sequence (5′–3′) | Translation initiation rate |
|---|---|---|
| RBS1-F | CGAAAATCCCTAAGGAGCTTAAGCATGGGCAGCAGCCATCACCATCATCACC | 90,504.51 |
| RBS1-R | GCTTAAGCTCCTTAGGGATTTTCGATTAAAGTTAAACAAAATTATTTCTACAGGGGAATTGTTATCCGCTC | |
| RBS2-F | CATCACCGTTAGACGAGGAGGTATCCTATGGGCAGCAGCCATCACCATCATCACC | 212,828.18 |
| RBS2-R | AGGATACCTCCTCGTCTAACGGTGATGATTAAAGTTAAACAAAATTATTTCTACAGGGGAATTGTTATCCGCTC | |
| RBS3-F | AATACCCTACTAAGGAGGTAAGCATGGGCAGCAGCCATCACCATCATCACC | 318,342.84 |
| RBS3-R | GCTTACCTCCTTAGTAGGGTATTATTAAAGTTAAACAAAATTATTTCTACAGGGGAATTGTTATCCGCTC | |
| RBS4-F | GAACTCGAACATAGTCTTAAGGAGGTTCAAATGGGCAGCAGCCATCACCATCATCACC | 370,978.54 |
| RBS4-R | TTGAACCTCCTTAAGACTATGTTCGAGTTCATTAAAGTTAAACAAAATTATTTCTACAGGGGAATTGTTATCCGCTC |
a
The sequences of RBS are underlined.