TABLE 3.
Kinetic parameters of MaiA and its variants
Enzyme | Km (mM) | kcat (min−1) | kcat/Km (min−1 · mM−1) |
---|---|---|---|
Wild type | 0.6 ± 0.02 | 19.1 ± 0.10 | 31.8 |
G27A/K104R | 1.1 ± 0.04 | 35.2 ± 1.56 | 32.0 |
G27A/G171A | 1.2 ± 0.04 | 51.6 ± 2.01 | 43.0 |
Kinetic parameters of MaiA and its variants
Enzyme | Km (mM) | kcat (min−1) | kcat/Km (min−1 · mM−1) |
---|---|---|---|
Wild type | 0.6 ± 0.02 | 19.1 ± 0.10 | 31.8 |
G27A/K104R | 1.1 ± 0.04 | 35.2 ± 1.56 | 32.0 |
G27A/G171A | 1.2 ± 0.04 | 51.6 ± 2.01 | 43.0 |