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. 2019 Sep 17;85(19):e01287-19. doi: 10.1128/AEM.01287-19

TABLE 5.

Strains and plasmids used in this study

Strain or plasmid Description Source
Strains
    Heliobacterium modesticaldum Ice1 (ATCC 51547) Originally isolated from hot springs soil in Iceland (8) Gift from Michael Madigan
    E. coli
        S17-1 Mobilization host containing chromosomally integrated tra genes ATCC 47055
        NEBα Cloning strain NEB
        TOP10 Cloning strain Invitrogen
Plasmids
    pBAD33 Low-copy-number vector containing arabinose-inducible promoter ATCC 87402
    pPB347 pBAD33 bearing HM1_2858, HM1_3075, HM1_3076, HM1_3037, and HM1_3004 This study
    pJIR1457 E. coli-C. perfringens shuttle vector Gift from Joseph A. Sorg (21)
    pMTL82251 Modular plasmid with pBP1 replication module Chain Biotech (23)
    pMTL83251 Modular plasmid with pCB102 replication module Chain Biotech (23)
    pMTL84251 Modular plasmid with pCD6 replication module Chain Biotech (23)
    pMTL85251 Modular plasmid with pIM13 replication module Chain Biotech (23)
    pMTL86251 Modular plasmid with pIP404 replication module Chain Biotech (23); this study