TABLE 6.
Oligonucleotide primers used in this study
| Primer name | Primer sequencea |
|---|---|
| HM1_2858 KpnI SD for | attcggtacctaacaggaggaattaaccATGGTTATGTTTACATCTGTTG |
| HM1_2858 PstI PacI rev | taatctgcagttaattaaTCACGCTCCTTTCATTTCAA |
| HM1_3076 KpnI PacI SD for | taatggtaccttaattaataacaggaggaattaaccATGGAACTGCTGGCCGAAg |
| HM1_3076 PstI BglII rev | acgtactgcagagatctCTATATCATTTTAGCGTATATGT |
| HM1_3075 KpnI BglII SD for | acgtggtaccagatcttaacaggaggaattaaccATGGCCGACAAACTGCGC |
| HM1_3075 PstI SpeI rev | acgtactgcagactagtTCAAAACTCATACCCAATCGC |
| HM1_3037 KpnI SpeI SD for | acgattggtaccactagttaacaggaggaattaaccGTGGATGAACTCGCCGTTGAAC |
| HM1_3037 PstI NdeI rev | acgtactgcagcatatgTTAGGCCGGACCGCAAAGGGAT |
a
First restriction sites are underlined, second restriction sites are double underlined, ribosome binding sites are italicized, and sequences annealing to the H. modesticaldum genome are capitalized.