TABLE 1.
Plasmid and strains used in this study
| Plasmid or strain | Characteristicsa | Reference |
|---|---|---|
| Plasmids | ||
| pCM351 | Genr Tetr; broad-host-range cre-lox vector | 57 |
| pJQ200SK | Genr; P15A origin from pACYC184; lacZ sacB traJ | 54 |
| pVO155 | Kmr; pUC119 derivative for insertion into genome | 56 |
| pBBR1MCS-2 | Kmr; broad-host-range vector | 58 |
| pBBR1MCS-3 | Tetr; broad-host-range vector | 58 |
| pRK2013 | Kmr; ColE1 replicon, tra+ from RK2 | 55 |
| pBBR1MCS-2-PphaC-eyfp-n1 | Kmr; universal vector for construction of fusions N terminal to eyfp under the control of the phaCAB promoter | 44 |
| pBBR1MCS-3-PphaC-eyfp-n1 | Tetr; universal vector for construction of fusions N terminal to eyfp under the control of the phaCAB promoter | This work |
| pBBR1MCS-2-PphaC-phaP1-eyfp | Kmr; N-terminal fusion of PhaP1 to eYFP | This work |
| pBBR1MCS-2-PphaC-phaP2-eyfp | Kmr; N-terminal fusion of PhaP2 to eYFP | This work |
| pBBR1MCS-2-PphaC-phaP3-eyfp | Kmr; N-terminal fusion of PhaP3 to eYFP | This work |
| pBBR1MCS-3-PphaC-phaP1-eyfp | Tetr; N-terminal fusion of PhaP1 to eYFP | This work |
| pBBR1MCS-3-PphaC-phaP2-eyfp | Tetr; N-terminal fusion of PhaP2 to eYFP | This work |
| pBBR1MCS-3-PphaC-phaP3-eyfp | Tetr; N-terminal fusion of PhaP3 to eYFP | This work |
| pBBR1MCS-2-PphaC-b11320-eyfp | Kmr; N-terminal fusion of NGR_b11320 to eYFP | This work |
| pBBR1MCS-2-PphaC-b13620-eyfp | Kmr; N-terminal fusion of NGR_b13620 to eYFP | This work |
| pBBR1MCS-2-PphaC-c33730-eyfp | Kmr; N-terminal fusion of NGR_c33730 to eYFP | This work |
| pBBR1MCS-2-PphaC-b19160-eyfp | Kmr; N-terminal fusion of NGR_b19160 to eYFP | This work |
| pJQ200SK-phbC2 | Genr; pJQ200SK carrying phbC2 for in situ complementation | This work |
| pJQ200SK-phaP3 | Genr; pJQ200SK carrying phaP3 for in situ complementation | This work |
| Strains | ||
| Escherichia coli DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rk− mk+) phoA supE44 λ− thi-1 gyrA96 relA1 | |
| Sinorhizobium fredii | ||
| NGR234 | Rifr; wild type | 53 |
| phbC1 strain | Rifr Genr; NGR234 ΔphbC1::Gen | This work |
| phbC1C2 strain | Rifr Genr Kmr; NGR234 ΔphbC1::Gen with pVO155 inserted into phbC2 | This work |
| ΔphbC1 strain | Rifr; NGR234 with phbC1 precise deletion | This work |
| ΔphbC2 strain | Rifr; NGR234 with phbC2 precise deletion | This work |
| ΔphbC1C2 strain | Rifr; NGR234 with phbC1 and phbC2 precise deletion | This work |
| ΔphbC1C2+C2 strain | Rifr; ΔphbC1C2 strain complemented with phbC2 | This work |
| phaZ (NGR_b03370) strain | Rifr Kmr; NGR234 with pVO155 inserted into NGR_b03370 | This work |
| ΔphaP1 strain | Rifr; NGR234 with phaP1 precise deletion | This work |
| ΔphaP2 strain | Rifr; NGR234 with phaP2 precise deletion | This work |
| ΔphaP3 strain | Rifr; NGR234 with phaP3 precise deletion | This work |
| ΔphaP4 strain | Rifr; NGR234 with phaP4 precise deletion | This work |
| ΔphaP1P2 strain | Rifr; NGR234 with phaP1 and phaP2 precise deletions | This work |
| ΔphaP1P3 strain | Rifr; NGR234 with phaP1 and phaP3 precise deletions | This work |
| ΔphaP2P3 strain | Rifr; NGR234 with phaP2 and phaP3 precise deletions | This work |
| ΔphaP2P3+P3 strain | Rifr; ΔphaP2P3 strain complemented with phaP3 | This work |
| ΔphaP1P2P3 strain | Rifr; NGR234 with phaP1, phaP2, and phaP3 precise deletions | This work |
| ΔphaP1P2P3P4 strain | Rifr; NGR234 with phaP1, phaP2, phaP3, and phaP4 precise deletions | This work |
a
Genr, gentamicin resistance; Tetr, tetracycline resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance.