KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC α7-Integrin | AbLab | Cat#67-0010-05 |
| Alexa Fluor 555 α-Bungarotoxin | Invitrogen | Cat#B35451; RRID:AB_2617152 |
| APC β1-Integrin (CD29) | eBioscience | Cat#17-0291-82; RRID:AB_1210793 |
| PE-Cy7 CD31 (PECAM) | BD Biosciences | Cat#561410; RRID:AB_10612003 |
| Biotin CD34 | eBioscience | Cat#13-0341-81; RRID:AB_466424 |
| PE-Cy7 CD45 | BD Biosciences | Cat#552848; RRID:AB_394489 |
| Biotin CXCR4 | eBioscience | Cat#13-9991-82; RRID:AB_10609202 |
| DYSTROPHIN | Abcam | Cat#ab15277; RRID:AB_301813 |
| EGFP | Abcam | Cat#ab13970; RRID:AB_300798 |
| Embryonic myosin heavy chain | Developmental Studies Hybridoma Bank | Cat#F1.652; RRID:AB_528358 |
| Laminin | Sigma | Cat#L9393; RRID:AB_477163 |
| MHC-I | Developmental Studies Hybridoma Bank | Cat#BA-D5; RRID:AB_2235587 |
| MHC-IIa | Developmental Studies Hybridoma Bank | Cat#SC-71; RRID:AB_2147165 |
| MHC-IIb | Developmental Studies Hybridoma Bank | Cat#BF-F3; RRID:AB_2266724 |
| MYOD1 | Santa Cruz Biotechnology | Cat#sc-304; RRID:AB_631992 |
| PAX7 | Developmental Studies Hybridoma Bank | Cat#PAX7; RRID:AB_528428 |
| Sarcomeric MHC | Developmental Studies Hybridoma Bank | Cat#MF-20; RRID:AB_2147781 |
| Biotin VCAM-1 | BD Biosciences | Cat#553331; RRID:AB_394787 |
| Streptavidin-PE | BD Biosciences | Cat#554061; RRID:AB_10053328 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| KO-DMEM | Life Technologies | Cat#10829-018 |
| DMEM, high glucose | HyClone | Cat#SH30081.01 |
| DMEM/F12 | Cellgro | Cat#15-090-CV |
| FBS | PEAK serum | Cat#PS-FBS |
| ES cell-qualified FBS | Gemini Bio-Products | Cat#100-119 |
| Horse serum | HyClone | Cat#SH30074.03 |
| Non-essential amino acids | Life Technologies | Cat#11140-050 |
| Penicillin/streptomycin | Life Technologies | Cat#15140-122 |
| Glutamax | Millipore | Cat#SCR006 |
| β-Mercaptoethanol | Sigma | Cat#M3148 |
| Leukemia inhibitory factor | Millipore | Cat#ESG1107 |
| Chick embryo extract | US Biological | Cat#C3999 |
| Insulin-transferrin-selenium | Life Technologies | Cat#41400045 |
| Basic FGF | R&D Systems | Cat#233-FB/CF |
| Propidium iodide | Sigma | Cat#P4170 |
| Paraformaldehyde | Sigma | Cat#P6148 |
| Triton X-100 | Sigma | Cat#X100 |
| Bovine serum albumin | Fisher Bioreagents | Cat#BP1605-100 |
| PBS | HyClone | Cat#SH30256.01 |
| Gelatin | Sigma | Cat#G2500 |
| 0.25% Trypsin-EDTA | Life Technologies | Cat#25200-072 |
| DAPI | Life Technologies | Cat#D3571 |
| OCT solution | Scigen | Cat#4586 |
| 2-Methylbutane | Sigma | Cat#320404 |
| Acetone | Sigma | Cat#179124 |
| DAKO Retrieval Solution | Agilent | Cat#S169984-2 |
| Immu-Mount | Thermo Scientific | Cat#9990402 |
| Cardiotoxin | Sigma-Aldrich | Cat#C9759 |
| Ketamine (VetaKet) | Akorn | NDC:59399-114-10 |
| Xylazine (AnaSed) | Akorn | NDC:59399-111-50 |
| Avertin (tribromoethanol) | Sigma | Cat#T48402 |
| EdU | Invitrogen | Cat#A10044 |
| NaCl | Fisher Bioreagents | Cat#BP358-212 |
| NaHCO3 | Sigma | Cat#S7277 |
| Glucose | Sigma | Cat#G7021 |
| KCl | Fisher Bioreagents | Cat#BP366-500 |
| CaCl2 | Sigma | Cat#21097 |
| MgSO4 | Sigma | Cat#M7506 |
| NaH2PO4 | Sigma | Cat#S8282 |
| Sodium pyruvate | Sigma | Cat#P5280 |
| GoTaq Flexi DNA polymerase | Promega | Cat#M8298 |
| Premix Ex Taq (probe qPCR) master mix | Clontech | Cat#RR39WR |
| AluI | New England BioLabs | Cat#R0137S |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | Qiagen | Cat#74106 |
| Verso cDNA Synthesis Kit | Thermo Scientific | Cat#AB1453A |
| SMARTer Stranded Total RNA-Seq Kit – Pico Input Mammalian Kit | Clontech | Cat#634411 |
| Click-iT EdU Alexa Fluor 555 Imaging Kit | Invitrogen | Cat#C10338 |
| GeneJET Genomic DNA Purification Kit | Thermo Scientific | Cat#K0721 |
| Deposited Data | ||
| RNA-seq | This paper | GEO:GSE92892 |
| ES-Bruce4 RNA-seq | www.encodeproject.org | GEO:GSE93453 |
| Hematopoietic multipotent progenitor cell RNA-seq | www.encodeproject.org | GEO:GSE90209 |
| Experimental Models: Cell Lines | ||
| Mouse embryonic fibroblasts | This paper | N/A |
| E14-EGFP mouse ES cells | Ismailoglu et al., 2008 | N/A |
| C57BL/6N-PRX-B6N #1 mouse ES cells | The Jackson Laboratory (via Mouse Genetics Laboratory, University of Minnesota) | Stock#012448 |
| Pax7-ZsGreen mouse iPS cells | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| NSG-mdx4Cv mice | Arpke et al., 2013 | N/A |
| Pax7-ZsGreen mice | Bosnakovski et al., 2008 | N/A |
| 129P2/OlaHsd mice | Envigo | N/A |
| Oligonucleotides | ||
| Taqman qPCR assay: Gapdh | Applied Biosystems | Mm99999915_g1 |
| Taqman qPCR assay: Myf5 | Applied Biosystems | Mm00435125_m1 |
| Taqman qPCR assay: Myod1 | Applied Biosystems | Mm00440387_m1 |
| Taqman qPCR assay: Pax3 | Applied Biosystems | Mm00435491_m1 |
| Taqman qPCR assay: Pax7 | Applied Biosystems | Mm00834079_m1 |
| SCID PCR forward primer | GGA AAA GAA TTG GTA TCC AC | N/A |
| SCID PCR reverse primer | AGT TAT AAC AGC TGG GTT GGC | N/A |
| Recombinant DNA | ||
| pMXs-Oct3/4 | Addgene | Plasmid#13366 |
| pMXs-Sox2 | Addgene | Plasmid#13367 |
| pMXs-Klf4 | Addgene | Plasmid#13370 |
| Software and Algorithms | ||
| ImageJ (v2.0.0-rc-65/1.52a) | NIH | https://imagej.nih.gov/ij/ |
| FlowJo (v7.6.3) | FLOWJO LLC | https://www.flowjo.com/ |
| Prism (v6.07) | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ZEN (v2.3 pro) | Zeiss | https://www.zeiss.com/ |
| LabChart (v6.1.1) | ADInstruments | https://www.adinstruments.com/products/labchart/ |
| FACSDiva (v6.1.3) | BD Biosciences | http://www.bdbiosciences.com/ |