KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| 53BP1 | Millipore | Cat# MAB3804, RRID:AB_2256673 |
| Anti-53BP1 | Novus | Cat# NB100–304, RRID:AB_10003037 |
| BRCA1 (D-9) | Santa Cruz | Cat# sc-6954, RRID:AB_626761 |
| H2AX | Bethyl labs | Cat# A300–083A, RRID:AB_203289 |
| HA (clone HA-7) | Sigma-Aldrich | Cat# H9658, RRID:AB_260092 |
| γ-H2AX (Ser 139) clone JBW301 | Millipore | Cat# 05–636, RRID:AB_309864 |
| γ-H2AX | Cell Signaling Tech. | Cat# 2577, RRID:AB_2118010 |
| Phospho RPA32 (S4/S8) | Bethyl labs | Cat# A300–245A, RRID:AB_210547 |
| RAD51 (H92) | Santa Cruz | Cat# sc-8349, RRID:AB_2253533 |
| RAD51 | B-Bridge International | Cat# 70–001, RRID:AB_2177110 |
| RIF1 | Bethyl | Cat# A300–244A, RRID:AB_185548 |
| RPA2 | Bethyl | Cat# A300–245A, RRID:AB_210547 |
| LMO2 | Natkunam et al., 2008 | N/A |
| GAPDH | Santa Cruz | Cat# sc-25778, RRID:AB_10167668 |
| CD20 | Agilent | Cat# M0755, RRID:AB_2282030 |
| γ-Tubulin | Sigma-Aldrich | Cat# T5326, RRID:AB_532292 |
| Actin | Sigma-Aldrich | Cat# A2066, RRID:AB_476693 |
| 488 Fluor Donkey anti-Rabbit antibody | ThermoFisher Sc. | Cat# A-21207, RRID:AB_141637 |
| 488 Fluor Donkey anti-mouse antibody | ThermoFisher Sc. | Cat# A-21202, RRID:AB_141607 |
| 594 Fluor Goat anti-Mouse antibody | ThermoFisher Sc. | Cat# A-11020, RRID:AB_2534087 |
| 594 Fluor Goat anti-Rabbit antibody | ThermoFisher Sc. | Cat# A-11012, RRID:AB_2534079 |
| 647 Fluor Donkey anti-goat antibody | ThermoFisher Sc. | Cat# A-21447, RRID:AB_2535864 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP | ThermoFisher Sc. | Cat# A16110, RRID:AB_2534782 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP | ThermoFisher Sc. | Cat# A16078, RRID:AB_2534751 |
| Biological Samples | ||
| Patient Derived Xenografts (PDXs) | Chapuy et al., 2016 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Olaparib | LC Labs | Cat# O9201 |
| BrdU | Sigma-Aldrich | Cat# B5002 |
| Doxycycline | Sigma-Aldrich | Cat# D9891 |
| Demecolcine | Sigma-Aldrich | Cat# D7385 |
| Permount | Electron Microscope Sciences | Cat# 17986–01 |
| DAPI | Millipore-Sigma | Cat# 268298 |
| CometAssay®Lysis Solution | Trevigen | Cat# 4250–010-01 |
| Bacterial and Virus Strains | ||
| pLKO.1 shLMO2–1 | Dharmacon Inc. | TRCN0000017136 |
| pLKO.1 shLMO2–2 | Dharmacon Inc. | TRCN0000017132 |
| pLKO.1 sh53BP1–1 | Dharmacon Inc. | TRCN0000018866 |
| pLKO.1 sh53BP1–2 | Dharmacon Inc. | TRCN0000018869 |
| Critical Commercial Assays | ||
| CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) | Promega | Cat# G5421 |
| SuperSignal™ West Femto Maximum Sensitivity Substrate | ThermoFisher Sc. | Cat# 34095 |
| Halt™ Protease and Phosphatase Inhibitor Cocktails | ThermoFisher Sc. | Cat# 78441 |
| Slowfade Diamond antifade reagent | ThermoFisher Sc. | Cat# S36963 |
| MethoCult | Stem Cell Technologies | Cat# H4100 |
| JetPrime Transfection Reagent | Polyplus Transfection | Cat# 114–07 |
| Experimental Models: Cell Lines | ||
| OCI-LY19 | Ontario Cancer Institute (OCI) | N/A |
| OCI-LY1 | Ontario Cancer Institute (OCI) | N/A |
| Doxycycline induced LMO2-GFP OCILY-1 | Gift from Ari Melnick Weill Cornell MC, NY |
N/A |
| OCI-LY7 | Ontario Cancer Institute (OCI) | N/A |
| OCI-LY8 | Ontario Cancer Institute (OCI) | N/A |
| DOHH2 | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC-47, RRID: CVCL_1179 |
| VAL | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC-586, RRID: CVCL_1819 |
| SUDHL6 | American Type Culture Collection (ATCC) | Cat# CRL-2959, RRID: CVCL_2206 |
| U2932 | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC-633, RRID: CVCL_1896 |
| Jurkat | American Type Culture Collection (ATCC) | Cat# TIB-152, RRID: CVCL_0367 |
| DND41 | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC 525, RRID: CVCL_2022 |
| MOLT16 | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC 29, RRID: CVCL_1424 |
| MOLT4 | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) | Cat# ACC 362, RRID: CVCL_0013 |
| HCC1187-BL | American Type Culture Collection (ATCC) | Cat# CRL-2323, RRID: CVCL_1248 |
| IMR90 | American Type Culture Collection (ATCC) | Cat# CCL-186, RRID: CVCL_0347 |
| HEK-293T | American Type Culture Collection (ATCC) | Cat# CRL-3216, RRID: CVCL_0063 |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.CB17-Prkdcscid/NCrHsd | Envigo | N/A |
| Mouse: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ | The Jackson Laboratory | Cat# 005557 |
| Oligonucleotides | ||
| ON-TARGETplus Human BRCA1 (672) siRNA - SMARTpool | Dharmacon | L-003461–00- 0010 |
| ON-TARGETplus Non-targeting Control Pool | Dharmacon | D-001810–10- 20 |
| Recombinant DNA | ||
| pDRGFP | Addgene | Cat# 26475 |
| pCBASceI | Addgene | Cat# 26477 |
| mcherry-LMO2 lentiviral vector | Genecopoeia | Cat# CS-A2951-Lv113 |
| pEGFP-C1-FLAG-Ku70 | Addgene | Cat# 46957 |
| pimEJ5GFP | Addgene | Cat# 44026 |
| Software and Algorithms | ||
| LASX software | Leica | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| CometScore IV | Instem | https://www.instem.com/ |
| Prism 6.0 | Graph Pad | https://www.graphpad.com/scientific-software/prism/ |
| CalcuSyn software | Biosoft | http://www.biosoft.com/w/calcusyn.htm |
| FlowJo | FlowJo, LLC | https://www.flowjo.com/ |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |