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. Author manuscript; available in PMC: 2019 Sep 19.
Published in final edited form as: Sci Transl Med. 2018 Oct 24;10(464):eaat3487. doi: 10.1126/scitranslmed.aat3487

Fig. 3. NF-κB is activated by DKK3 in PSCs and PDAC cells and is necessary for DKK3-mediated stimulation of cell activity.

Fig. 3.

(A) Phosphorylation of p65 and IκBα induced by DKK3 treatment (10 μg/ml) was determined by Western blotting. Relative protein loading was shown by using anti–β-actin Ab. (B) Western blot showing the time course of p65 activation in HPSC and Panc1 cells. Cells were treated with recombinant DKK3 (10 μg/ml) for 0 to 24 hours, and changes in band density relative to baseline were quantified. (C) DKK3 stimulates NF-κB luciferase reporter in HPSC and PDAC cells, compared to the mutant luciferase reporter (MT). NF-κB activity induced by DKK3 was measured in Panc28 with phosphorylation-defective IκBαM by (D) luciferase reporter and (E) Western blotting. (F) Proliferation of Panc28 and Panc28/IκBαM was measured by MTT assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus PBS control.