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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Small. 2019 Apr 29;15(23):e1805510. doi: 10.1002/smll.201805510

Figure 20.

Figure 20

Bioprinting of cardiac tissues. (A) Schematic representation of seeding cardiomyocytes onto a scaffold containing endothelial cells to obtain an endothelialized myocardium. (B) Immunofluorescence staining of sarcomeric α-actinin (red) and Cx43 (green) of cardiomyocytes seeded on bioprinted microfibrous scaffolds with different aspect ratios of unit grids. (C) Magnified images showing the sarcomeric banding. (D) Beating analysis of the cardiac organoid on bioprinted scaffolds with different aspect ratios of unit grid. Reproduced with permission from ref.[234]. (E) Schematic representation of a bioprinting process based on nanofiber yearns to obtain 3D engineered cardiac anisotropy. (F) 3D views of fluorescent images of cardiomyocytes on NFYs-NET scaffolds by staining for f-actin (green) and nuclei (blue) after 5 days of cultivation. (G) 3D view in confocal images of GFP-endothelial cells (ECs, green) and cardiomyocytes (CMs, red) within scaffolds and the quantitative analysis of cellular orientation distribution of GFP-ECs and CMs, as well as the fluorescence intensity analysis for the cross-section of this cell-laden scaffold showing the distribution of ECs in hydrogel and CMs on NFYs-NET. Reproduced with permission from ref.[235].