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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Small. 2019 Apr 29;15(23):e1805510. doi: 10.1002/smll.201805510

Figure 6.

Figure 6

Freeform embedded bioprinting of different shapes and constructs. (A) Schematic showing extrusion of a supramolecular HA-Ad guest bioink into a HA-CD host matrix. (B) Rhodamine-labeled filaments extruded into a fluorescein-labeled support hydrogel from using different needle gauges. (C) Confocal images of a filament of a fluorescein-labeled bioink and then a continuous spiral of a second, rhodamine-labeled bioink extruded into an unlabeled support hydrogel. (D) Confocal images of discrete pockets of a rhodamine-labeled bioink extruded into an unlabeled support hydrogel. (E) Confocal images of mesenchymal stem cells (green) printed within a bioink into a support hydrogel containing NIH/3T3 fibroblasts (red). Reproduced with ref.[144]. (F) Model and bioprinted structure of a human right coronary arterial based on a 3D MRI image with perfusable structure. (G) Model of a human femur and printed design with alginate bioink after removal from the support hydrogel. (H, I) Bioprinted scaffolds with complex internal and external architectures based on 3D imaging data from whole organs for (H) brain and (I) heart. Reproduced with permission from ref.[145]. (J) Thin-shell octopus bioprinted in different designs and a model of a Russian doll with several layers bioprinted in the support hydrogel. (K) A continuous network of hollow vessels with features spanning several orders of magnitude in diameter and aspect ratio. Reproduced with permission from ref.[146].