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The SEC-RPLC-CZE-MS/MS system for large-scale top-down proteomics of E. coli cells [73]. Proteoforms extracted from E. coli cells were fractionated by SEC to 5 fractions. Each SEC fraction was further separated by RPLC into 20 fractions. All the fractions were analyzed by CZE-MS/MS and the data were processed by the TopPIC (Top-down mass spectrometry based Proteoform Identification and Characterization) software [27] for proteoform IDs. Reproduced with permission [73].
