Fig 1. Generation of (hCB)-CD34⁺ NSG mice and propagation of (hCB)-CD34⁺ cells.

NSG recipient mice (n = 9) were irradiated (1 Gy) prior to transplantation. Engraftment of human cells has been evaluated via detection of human CD45⁺ cells in the PB of (hCB)-CD34⁺ humanized NSG mice (flow cytometry analysis) a) engraftment at 7 weeks post-transplant of (hCB)-CD34⁺ cells in 3/9 transplanted mice (percentage of detected hCD45⁺ cells is 29.2±13.1) b) engraftment at 6 weeks post-transplant of BM from P1 (hCB)-CD34⁺ humanized NSG mice in 3/3 transplanted mice (percentage of detected hCD45⁺ cells is 18.7±2.3) c) engraftment at 16 weeks post-transplant of BM from P2 (hCB)-CD34⁺ humanized NSG mice in 0/4 transplanted mice (hCD45⁺ cells were not detected in any of the transplanted mice).