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. 2019 Sep 19;15(9):e1007996. doi: 10.1371/journal.ppat.1007996

Fig 4. Characterization of the smooth PVP94/07 and bumpy surfaced PVP103/07 DENV2 clinical isolates.

Fig 4

(A-E) Neutralization profiles of MAbs (A) 4G2, (B) 4.8A, (C) 1A1D-2 (D) 2D22 and (E) C10 against PVP94/07 and PVP103/07. Data points represent average of n = 3 experiments with SD error bar. (F) Comparison of the attachment ability of PVP103/07 and PVP94/07 to C6/36 and HuH-7 cells. Y-axis shows genome copies level relative to PVP94/07, details of the formula is in the methods section. More bumpy surface particles (PVP103/07) are observed to bind to HuH-7 than the smooth surfaced PVP94/07 while no significant difference is detected when these virus are exposed to C6/36. Each data point represents one independent experiment with error bar representing SD. (* p<0.05) (G) Fusion assay of PVP94/07 and PVP103/07 with liposomes showed that bumpy surfaced PVP103/07, has a better fusion efficiency to liposomes. The average with standard error calculated from averaging the replicates of three individual experiments each with triplicates is presented. A two-way ANOVA followed with Fisher’s LSD test was carried out to compare the extent of fusion (%) of DENV2 PVP94/07 with DENV2 PVP103/07 at pH 5.0. ***p = 0.0010 shows the fusion efficiency is significantly different between PVP94/07 and PVP103/07.