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. 2019 Sep 19;14(9):e0223022. doi: 10.1371/journal.pone.0223022

Fig 7. Profiles of density of HCV produced in different hepatoma cell lines.

Fig 7

HuH-7 (A), Hep3B (B) and PLC/PRF/5 (C) were electroporated with in vitro transcribed RNA of the JFH1-CS-A4-RLuc genome containing mutations R1373Q/C2441S. The supernatants of each electroporated cell lines were recovered six days post-electroporation and overlaid on 10 to 50% (weight/volume) iodixanol gradients. After a 24 h ultracentrifugation, sixteen fractions were collected and analyzed for HCV RNA quantity and infectivity on naive HuH-7 cells (assessed by measuring Renilla Luciferase activities). The results are expressed as percentages of total infectivity or HCV RNA and are reported as means of two independent experiments.