Fig 3. Involvement of AP-1 in the regulation of Tspo expression by LPS stimulation.

(A) Schematic of the predicted and established transcription factor binding sites upstream of the mouse Tspo gene. The blue letters indicate established transcription factors that regulate the mouse Tspo genes based on a previous study. TSS: Transcriptional start site, UTR: Untranslated region (B) Forty-eight hours after transfection of the indicated vectors, BV-2 cells were treated with 100 ng/ml LPS for an additional 8 hours. Luciferase activity was measured as described in the Materials and methods section. The asterisks indicate statistically significant differences (n = 4, **p<0.01).