Fig 2. The identification of emerging and re-emerging virus-derived peptides binding to Ptal-N*01:01.

The binding of peptides derived from HeV (A), EBOV (B), MERS-CoV (C), and H17N10 influenza-like virus (H17N10) (D) with Ptal-N*01:01 were evaluated by co-refolding. Co-refolding without any peptide was termed as the negative control (No pep), as curves in gray color. After properly refolding, the high-absorbance peaks of the correctly refolded MHC I with the expected molecular mass of 45 kDa were eluted at the estimated volume of 16 mL on a Superdex Increase 200 10/300 GL column. The profile is marked with the approximate positions of the molecular mass standards of 75.0, 44.0, and 13.7 kDa. Inset, reduced SDS–PAGE gel (15%) of Ptal-N*01:01/HeV1 complex for peak 1 (P1), peak 2 (P2), and peak 3 (P3). Lane M contains molecular-mass markers (labeled in kDa). P1, P2, and P3 represent the aggregated heavy chain, the correctly refolded heterotrimer Ptal-N*01:01 complex (45 kDa), and the extra β₂m, respectively. β₂m, β₂-microglobulin; HeV, Hendra virus; EBOV, Ebola virus; MERS-CoV, Middle East respiratory syndrome coronavirus; MHC, major histocompatibility complex; P1, peak 1; P2, peak 2; P3, peak 3.