Fig 4. Assessment of peritumoral and intratumoral collagen deposition in paraffin tumor sections.

The collagen content of the tumors was analyzed by Masson´s trichrome histochemical staining in control and treated groups. A grading system composed of mild/focal (grade 1), moderated (grade 2) and extensive (grade 3) collagen deposition was used. The columns represent the mean ± standard error of the mean (SEM), of the score values corresponding to peritumoral collagen (white) and intratumoral collagen (grey) of the tumor sections corresponding to each treatment group. The statistically analysis by One-Way ANOVA shows no statistical significant difference between groups. (One-Way ANOVA, Dunnett's multiple comparison test P>0.05).