Table 1. Quantitative analysis of the morphological phenotypes displayed by 231/LM2-4 multicellular structures grown in a 3D lrECM “on-top” assay using Matrigel as barrier.

		3D Morphology
Group	Mass	Pseudo-Stellate Mass	Stellate	Structuresnumber	x10 fields analyzed
Control	49 (33.8%)	10 (6.9%)	86 (59.3%)	145	56
5-FU	159 (61%)	46 (17.6%)	56 (21.4%)	261	88
4-HC	131 (59.5%)	44 (20%)	45 (20.4%)	220	95
5-FU + 4-HC	136 (53.3%)	46 (18%)	73 (28.6%)	255	101

The 231/LM2-4 cells were culture without drugs (Control) or with 1μM 5-FU, 0.01μM 4-HC or the combination 1μM 5-FU + 0.01μM 4-HC for 6 days. The medium was changed every single day for six days. Chi-square analysis show statistical difference for control versus 5-FU, 4-HC and 5-FU+4-HC (p<0.05). No significant difference was found with comparisons involving 5-FU, 4-HC and 5-FU + 4-HC (Chi-square analysis; p>0.05).