Figure 6.

Rosette size and the percentage of unhealthy tissue in 10 single ago mutant plants infected with TCV at 17 dpi. A, Representative visualization of rosette leaves and the corresponding pseudo-color images in Col-0, dcl2 dcl3 dcl4, and 10 single ago mutant plants, which were separately inoculated by mock solution, TCV, or TCV CPB inoculum. Photos were taken individually at 17 dpi, and digitally extracted and aligned for comparison. In the pseudo-color images, green color referred to healthy plant pixels; red color referred to chlorotic/necrotic (unhealthy) plant pixels. B, The box plot shows the rosette area (total pixel number) in different genotypes at 17 dpi. C, The box plot shows the percentage of unhealthy tissue (red pixels/total pixels) in different genotypes at 17 dpi. Statistical analysis was calculated between treatments in each genotype. Boxes with different letters are statistically different (n = 4, Tukey post hoc test with α = 0.05).