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Plots of initial velocity versus substrate concentration for recombinant VvLAR1 and VvLAR2 with Cys-C and Cys-EC as substrates. The enzyme reactions were performed in a total volume of 100 μL containing 250 μm NADPH, 4 μg VvLAR1 or VvLAR2, and indicated amounts of Cys-C or Cys-EC with 10 min incubation. The products were quantified using (+)-catechin as standard on HPLC at 280 nm. The experimental points are presented as the average of three technical replicates with sd as error bars.
