Table 1. Kinetic parameters for VvLAR1 and VvLAR2 with Cys-C and Cys-EC as substrates.

Kinetic parameters were determined by fitting the initial velocity data to the Michaelis-Menten equation by nonlinear regression analysis using GraphPad Prism 7 software. Experimental data are presented in Figure 4.

Enzyme	Substrate	Vmax (nmol min−1 mg−1)	Km (μm)	Kcat (min−1)	Kcat/Km (min−1 M−1)
VvLAR1	Cys-EC	9.37 ± 0.43	30.49 ± 3.91	0.74	2.43 × 104
VvLAR2	Cys-EC	6.49 ± 0.28	16.80 ± 2.46	0.53	3.15 × 104
VvLAR1	Cys-C	48.12 ± 2.66	61.21 ± 10.06	3.89	6.36 × 105
VvLAR2	Cys-C	38.01 ± 1.53	42.40 ± 5.71	3.10	7.31 × 105