Table 2. Identification of Rossmann folds and prediction of cofactor specificity for wild-type and mutant VvLAR2 proteins.

The amino acids used in the analysis are wild-type LAR2 from 'Cabernet Sauvignon' (VvLAR2-CS), mutagenized proteins of VvLAR2-CS (VvLAR2-CS/K218E and VvLAR2-CS/V25E), LAR2 from 'Shiraz' (VvLAR2-Sh), and LAR1 from 'Cabernet Sauvignon' (VvLAR1-CS), which served as a positive control. Each identified Rossmann fold sequence domain is associated with three neural network scores calculated by the Cofactor V.1.0 server (Geertz-Hansen et al., 2014). A score above 0.5 indicates that the domain is predicted to be specific for the particular cofactor. The prediction scores are followed by a summary of the predicted cofactor specificity. Multiple specificities are separated with a slash. The approximate Rossmann fold sequence boundaries are provided next to the summary.

Sequence	Domain	FAD	NAD	NADP	Cofactor(s)	From	To
VvLAR2-CS	1	0.149	0.362	0.757	NADP	19	64
	2	0.000	0.616	0.121	NAD	62	113
VvLAR2-CS/K218E	1	0.149	0.362	0.757	NADP	19	64
	2	0.000	0.616	0.121	NAD	62	113
VvLAR2-CS/V25E	1	0.027	0.239	0.498	–	21	113
VvLAR2-Sh	1	0.027	0.239	0.498	–	21	113
VvLAR1-CS	1	0.227	0.538	0.541	NAD/NADP	10	51
	2	0.000	0.129	0.037	–	50	80