Figure 6.

Direct binding of TF to promoter fragment. FLAG-tagged TF proteins and biotinylated and unbiotinylated DNA probes were used in AlphaScreen assays to detect direct binding in vitro. Detected signals were normalized with those of the control samples with the corresponding unbiotinylated probes. A gene name in the probe ID indicates the source promoter of the probes. Relative AlphaScreen signals were calculated as the ratio of biotinylated-probe signal to unbiotinylated-probe signal. The values represent the averages and ses of triplicate experiments. A vertical dotted line shows a threshold value (relative AlphaScreen signal = 10.0). The positive control shows assays of the RRTF1 protein and the target sequence probe (Matsuo et al., 2015). The negative control shows assays with no template control of protein synthesis.