The O-GlcNAcylated and phosphorylated peptides of TaGRP2 identified by MS. A, The amino acid sequences of TaGRP2 with identification of phosphorylation and O-GlcNAcylation modification sites. B, The representative MS/MS spectra of O-GlcNAcylated peptide. C, The representative MS/MS spectra of phosphorylated peptide. D, Three-dimensional structure map of TaGRP2 predicted by Phyre; the phosphorylated site is S152, and the O-GlcNAcylated site is S87 (http://www.sbg.bio.ic.ac.uk/phyre2/). E, The estimated accuracy of the predicted 3D structure of TaGRP2.