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. 2019 May 6;180(3):1436–1449. doi: 10.1104/pp.19.00081

Figure 6.

Figure 6.

Mutant of S87 or S152 affects the TaGRP2’s binding to TaVRN1-RIP3. A, RNA-EMSA assay to analyze the binding of GRP2 and GRP2 mutants to the TaVRN1-RIP3. T17m, S87m, and S152m mean the GRP2 protein with mutation of T17, S87, and S152, respectively; SSm2 means GRP2 protein with mutation of S87 and S152; TSSm3 means GRP2 protein with mutation of T17, S87, and S152. The numbers above indicate the average band intensity of three replicates as quantified using Image J. The Coomassie brilliant blue (CBB) signal was normalized by EMSA signal. B, CBB staining result of the EMSA samples in (A). C, The quantitative data of three replicates for the binding-affinity comparisons among wild-type and mutant GRP2 proteins. Data shown are means ± sd; n = 3.