Stl1 proteins of Chlamydomonas sp. UWO241 are aligned with those of C. subellipsoidea, C. reinhardtii, and V. carteri. The color of the residue depends on the level of conservation among species (white, fully conserved; yellow, 80–100% similar; purple, 60–80% similar; blue, <60% similar). Regions corresponding to the putative signal peptide and kinase domain are indicated. The position of a putative Stt7-dependent phosphorylation pattern is marked with a green box, and Threonine167, the position of a Stt7-dependent phosphorylation site in C. reinhardtii (Lemeille et al., 2010), is highlighted in red. Both Stl1 isoforms from UWO241 lack this phosphorylation pattern.