Table 2. Gaussian Parameters for the Subband Decomposition of Low-Temperature (77K) Chlorophyll Fluorescence Emission Spectra of Clamydomonas sp. UWO241 Grown in LSand HS Media and Exposed to Light and Dark Treatments.
The percentage areas of the spectral forms have been calculated from the total area given by the sum of all bands. The FWHM of each band is the sum of the left and right HWHM values. PSII/PSI represents the ratio between the areas of PSII-related peaks (λ1, λ2, and λ3) and the area of the PSI peak (λ4). Mean values ± se were calculated from six to nine measurements in three to four independent experiments.
Parameters | LS | HS | ||
---|---|---|---|---|
Dark | Light | Dark | Light | |
1 λ max | 675.5 | 679.2 | 682.9 | 679.7 |
FWHM | 3.96 | 8.50 | 10.59 | 9.32 |
Area % | 0.10 | 4.44 | 32.90 | 7.19 |
2 λ max | 682.8 | 684.2 | 689.1 | 683.9 |
FWHM | 10.15 | 9.55 | 3.02 | 10.24 |
Area % | 18.12 | 9.49 | 0.06 | 24.46 |
3 λ max | 694.3 | 694.8 | 694.4 | 694.7 |
FWHM | 14.43 | 13.59 | 13.57 | 13.70 |
Area % | 20.38 | 18.43 | 23.87 | 23.20 |
4 λ max | 707.3 | 707.0 | 705.8 | 706.4 |
FWHM | 26.09 | 26.12 | 24.76 | 24.86 |
Area % | 42.82 | 42.42 | 26.98 | 35.76 |
5 λ max | 731.6 | 732.9 | 736.4 | 734.6 |
FWHM | 44.85 | 44.95 | 43.58 | 42.50 |
Area % | 18.55 | 18.49 | 16.46 | 18.27 |
PSII/PSI | 0.90 | 0.76 | 2.10 | 1.53 |