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. 2019 May 13;180(3):1677–1690. doi: 10.1104/pp.19.00391

Figure 1.

Figure 1.

Expression patterns of NF-YB2 and NF-YB3 in ABA signaling, ABA-deficient, and HSFA1 knockout mutants (hsfa1a/b/d) during dehydration and heat stress. A and B, Expression levels of NF-YB2, NF-YB3, and DREB2A during dehydration stress in the ABA-signaling mutants (A) and ABA-deficit mutants (B). The expression of NF-YB2, NF-YB3, and DREB2A genes was analyzed in the SRK2D triple mutants (srk2d/e/i) and ABSCISIC ACID RESPONSIVE ELEMENTS-BINDING PROTEIN/FACTORs quadruple mutants (arebq; A) and NINE-CIS-EPOXYCAROTENOID DIOXYGENASE3 (NCED3; nc3-2) and ABA DEFICIENT2 (ABA2; aba2-2) knockout mutants (B) by reverse transcription quantitative PCR (RT-qPCR. The 14-d-old seedlings grown on agar plates were treated with dehydration stress. The expression levels of NF-YB2, NF-YB3, and DREB2A in wild-type plants before stress treatment (0 h) were defined as 1.0. The error bars indicate SD (n = three experiments, involving 7 individual plants for each line). The asterisk indicates the time point at which the expression levels of each gene were significantly decreased compared with those in the wild-type plants (P < 0.05, Bonferroni-corrected Student’s t test). C, The expression levels of NF-YB2, NF-YB3, and DREB2A during heat stress in the hsfa1a/b/d mutants. The 14-d-old seedlings grown on agar plates were treated with heat stress (37°C). The expression levels of NF-YB2, NF-YB3, and DREB2A in the wild-type plants before stress treatment (0 h) were defined as 1.0. The error bars indicate SD (n = three experiments, involving 7 individual plants for each line). The asterisk indicates the time point at which the expression levels of each gene were significantly decreased compared with that of both wild-type plants (Col and Ws; P < 0.05, Bonferroni-corrected Student’s t test).