Figure 6.

Expression patterns of dehydration-inducible and heat-inducible DREB2A target genes during dehydration and heat stress. A and B, Expression analysis of dehydration-inducible (A) or heat-inducible (B) DREB2A target genes during dehydration and heat stress in the nf-yb2, nf-yb3, and nf-yb2 nf-yb3 knockout plants. The expression levels of DREB2A and dehydration-inducible (LEA7, RD29B, LEA76, and CYSTM1) and heat-inducible (HSFA3, At1g75860, HSP70B, and At1g53540) DREB2A target genes during dehydration and heat stress were analyzed by RT-qPCR. The 14-d-old plants grown on agar plates were treated with dehydration or heat stress. The expression levels of each gene in the VC plants under the control condition (0 h) were defined as 1.0. The error bars indicate SD (n = three experiments, involving 7 individual plants for each line). Asterisks indicate significant differences from the VC plants at each time point (P < 0.05, Bonferroni-corrected Student’s t test). Small values of HSP70B and At1g53540 (HSP20-like) gene expression under 5- and 10-h heat stress conditions were enlarged. The expression levels of DREB2A at each time point during dehydration and heat stress were evaluated using one-way ANOVA, but no significant differences were detected (P > 0.05).