Extended Data Fig. 3.
Fap is expression in mouse cardiac fibroblasts after injury.
(a) Immunohistochemistry co-staining cardiac Troponin (red) and FAP (green; left) and Vimentin (green; right) in adjacent sections from the left ventricle of a mouse treated with AngII/PE for 2 weeks. Fap and Vimentin are seen on the same fibroblasts (arrowheads). Representative images from 2 independent experiments with similar results (n=7 biologically independent animals). (b) Immunohistochemistry for Fap (green) in various organs/tissues after 1 week of AngII/PE treatment. Representative image of 3 biologically independent animals with similar results. (c) Masson’s trichrome stain for fibrosis (blue; top, center) and immunohistochemistry for Fap (green; bottom) in WT coronal heart sections 2 week after continuous AngII/PE treatment. Staining and immunohistochemistry performed in adjacent sections. Bottom insets: higher magnification of left ventricular free-wall. Representative image from 2 independent experiments with similar results (n=7 biologically independent animals). (d) Immunohistochemistry for Fap (green) in mouse models of cardiac injury. MI: myocardial infarction, TAC: transverse aortic constriction, DMD: Duchenne’s muscular dystrophy (mdx/mTRKO G2 mice). Scale bars = 100μm.