Extended Data Fig. 6.

FAP CAR T treatment does not affect perivascular fibrosis or other organs.

(a) Masson’s trichrome stain (blue; left, center) and FAP immunohistochemistry (green; right) on adjacent heart coronal sections 1 week after commencement of continuous AngII/PE treatment. FAP expression in present in interstitial, but not perivascular fibroblast (white arrowheads). Centered on vessel from Fig. 2c. Representative images of 2 independent experiments with similar results. (b) Picro-Sirius Red staining for perivascular fibrosis (black arrowheads, red) on heart coronal sections from mice treated for 4 weeks with either saline, AngII/PE, or AngII/PE + FAP CAR T cells. Representative images of 2 independent experiments with similar results. (c) H&E staining of various tissue sections from mice treated for 4 weeks with either saline, AngII/PE, or AngII/PE + FAP CAR T cells. Representative images of 3 independent experiments with similar results. Scale bars = 100μm.