Table 1,

Summary of observed time constants by spectroscopic techniques in apo YopH and YopH/ligand complexes.

Enzymes	Observed time constant	Loop open	Loop close	Detection method
Apo YopH	4 and 30 ns	4 ns#	4 ns#	1, #: 5
Apo YopH	~3 μs	~5 μs*	~7 μs*	2, *: 4
Apo YopH	23 μs	23 μs	800 μs	3
YopH/Arsinate	4 and 50 ns			1
YopH/PNCS	20 - 110 μs	130 μs	11 μs	2
Q357F/PNCS	35 - 250 μs	380 μs	26 μs	2
Q357A/PNCS	13 - 60 μs	100 μs	9 μs	2
YopH/peptide	550 μs	550 μs	50 μs	3

1. Fluorescence Anisotropy; ¹⁰ 2. Fluorescence T-jump (Observed values for E/PNCS are concentration dependent. Loop open/closed values are derived from a four-state model); ^12,13 3. NMR (all values are derived from a two-state model), ¹⁴ 4. UVRR (this work) and 5. MD simulations. ¹¹

pNCS is a YopH specific inhibitor structurally similar to the small molecule substrate pNPP.³⁹