Figure S6.

Overexpressing pORF32/pORF19 Mutants Does Not Significantly Affect KSHV DNA Replication or Gene Expression, Related to Figure 5

(A-B) Viral genome replication in cells overexpressing wild-type or mutant forms of pORF32 (A) or pORF19 (B). 293T-KSHV cells were transfected with corresponding expression plasmids or empty vector as control, and then induced with NaB and TPA to facilitate KSHV lytic replication. Total DNA was extracted from cells, and genome replication was determined by qPCR. (C-D) Viral RNA transcription in cells transfected with wild-type or mutant forms of pORF32 (C) or pORF19 (D). Total RNA was extracted from the same cells as (A-B). Viral RNA transcripts were quantified by RT-qPCR and presented as x-fold changes over RNA level of empty vector transfected cells. Data is mean ± SEM (n = 3 biologically independent samples).