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. 2019 Sep 19;9:13568. doi: 10.1038/s41598-019-49867-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mesothelin (MSLN) expression in colorectal cancer. (A) Kaplan-Meier analysis of overall survival in the metastatic setting according to MSLN expression levels quantified by mass spectrometry (MS). (B) Correlation between MS (x-axis, amol/ug) and immunohistochemistry (y-axis, H-score). Pearson correlation coefficient, 95% confidence interval and p-value are indicated. (C) Representative immunohistochemistry staining of a MS MSLN-negative (upper panel; ID-79, liver metastasis, H-score = 0) and –positive (lower panel; ID-72, primary CRC, H-score = 210) cases. Digital magnification: 0.5 × (left) and 20 × (right).