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. 2019 Sep 19;9:13567. doi: 10.1038/s41598-019-49729-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of S. flexneri lux1 calibration curves. The log transformed S. flexneri lux1 relative luminescence units (RLU) were plotted as a function of log transformed CFUs. The limit of detection was 7,500 CFUs with the Perkin Elmer Envision system (a). The experiment was repeated and analyzed with a Perkin Elmer IVIS Spectrum (b). With the IVIS Spectrum, the limit of detection was 47,315 CFUs. Linear regression was used to identify an association between the luminescence and CFUs. Each point represents the average of three technical replicates.