Fig. 4.
Polq is required for Mitomycin C induced DNA damage repair. a Metaphase aberrations and sister chromatid exchanges are shown in WT, Polq−/− and PolqhPOLQ cells 12 h after treatment with 20 ng/mL Mitomycin C (MMC). Scale bar = 10 µm. b, c Quantification of (a), 35 metaphase spreads for each condition was scored, and shown are mean ± SEM. Significance determined using an unpaired, two-tailed t-test (*p < 0.05; ****p < 0.0001). d IF analysis of WT, Polq−/−, and PolqhPOLQ cells six hours after treatment with 20 ng/mL MMC, stained with DAPI (blue) and antibodies specific for Rad51 (red) and 53BP1 (green) (n = 3 biologically independent experiments). (e, f) are quantification of (d). Statistical significance was assessed by unpaired, two-tailed Mann–Whitney tests (***p < 0.001; ****p < 0.0001)