Fig. 5.

Polq is required for Pyridostatin induced DNA damage repair. a Colony forming efficiency after treatment with Pyridostatin (PDS, 1, 2, 5 µM) in WT and Polq^−/− MEFs. b Colony forming efficiency after treatment with Pyridostatin (PDS, 1, 2, 5 µM) in WT MEFs transduced with Cas9 and either sgCtrl or sgPolq (targeting the polymerase domain). a, b Data shown are the mean ± SEM (n = 3). Statistical significance was assessed by two-tailed t-tests. *p < 0.05, **p < 0.01 and ***p < 0.001. c IF images for DAPI (blue), Rad51 (red) and 53BP1 (green) in WT and Polq^−/− MEFs six hours after treatment with 5 µM PDS (n = 3 biologically independent experiments). d, e are quantification of large Rad51 and 53BP1 foci, as observed in (c). Shown are mean ± SEM, and representative of three independent experimental replicates. Statistical significance was assessed by Mann–Whitney tests. **p < 0.01, ***p < 0.001, and ****p < 0.0001