Fig. 4.

The MACPF domain of MPEG1 is oriented away from the MABP-bound membrane. a Examples of MPEG1 bound to liposomes reveal b single rings bound in the prepore state (black arrows) and the occasional example of pores (i.e., the membrane is absent in the pore lumen), that coordinate a second liposome through the top portion of the ring (white arrow). We were not able to identify examples of pores bound to a single liposome, i.e. all pore structures we observed bridged between two liposomes. Class averages for prepores (a) and pores (d) are shown. For comparison, c a single pore, d a 2D class average and e illustration are shown