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. 2019 Sep 19;9:13555. doi: 10.1038/s41598-019-49929-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SLC29A4/hENT4 expression in PK15-NTD cells and SLC29A4 transfected PK15-NTD cells. (a) PCR: cDNA was prepared from Total RNA isolated from PK15-NTD cells and cells stably transfected with SLC29A4 (PK15-hENT4). The transcripts for transfected human SLC29A4 and endogenous pig GAPDH were amplified as described in the text. (b) Immunoblots: Membranes were prepared from PK15-NTD and PK15-hENT4 cells. Samples were resolved on SDS-PAGE gels, transferred to polyvinyl membranes and probed with anti-myc and anti-GAPDH antibodies to detect myc-tagged hENT4 and GAPDH (loading control), respectively. Complete gels are shown in Supplemental Fig. 1.