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. 2019 Sep 13;13:962. doi: 10.3389/fnins.2019.00962

FIGURE 2.

FIGURE 2

Hypothalamic phenotype at P21. (A–F) Representative Western Blots of INSR, LEPR, pAKT, pGSK3β, pAMPK, and IBA1 in hypothalamic tissue at P21. (A–F) Densitometric analysis of protein expression at P21: (A) INSR/GAPDH, CO n = 5, HFD n = 5. (B) LEPR/GAPDH, CO n = 5, HFD n = 5. (C) pAKT/AKT, CO n = 4, HFD n = 4. (D) pGSK3β/GSK3β, CO n = 5, HFD n = 4. (E) pAMPK/AMPK. (F) IBA1/GAPDH, CO n = 5, HFD n = 5. Data are presented as mean ± SEM. p < 0.05; ∗∗p < 0.01; CO, control; HFD, high fat diet.