Table 2.

Migratory capacity of CCR7 mutations.

	CCL21 (10 nM)		CCL19 (10 nM)
Mutation (position)	% Specific migration at 10 nM + SEM		% Specific migration at 10 nM + SEM
WT	5.1 ± 0.50		11.6 ± 1.58
R54A (N-term)	0.3 ± 0.37	*	−0.1 ± 0.08	*
L61A (1.35)	4.0 ± 0.49	*	7.5 ±1.94	ns
W114A (2.60)	0.6 ± 0.45	*	1.0 ±0.66	*
R209A (ECL2)	10.6 ± 1.00	*	5.0 ± 0.55	*

The specific migration of 300–19 pre-B-cells stably transfected with CCR7 wild type or mutant constructs in a Transwell migration assay is shown. Data are presented as the specific migration at 10 nM chemokine, at which concentration both CCL21 and CCL19 display peak activity, and values represent data presented in Figure 3. The specific migration is calculated as the percentage of specifically migrated cells relative to the input and shown as mean values (±SEM) from four independent experiments. Each mutant is compared to wildtype by ANOVA and asterisks identify significant differences, while “ns” refers to a non-significant change. Residue position refers to the location in CCR7 based on the nomenclature of Ballesteros-Weinstein, as shown in Table 1 (41). Bold values indicate main findings, italic values indicate statistical significance.