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Optimization of amplification temperature for MCDA primer sets. The MCDA assays for MTB detection were monitored by real-time measurement of turbidity. The threshold value was 0.1, thus turbidity of >0.1 was regarded to be positive. Eight kinetic graphs (1–8) were obtained at different temperatures (61 –68°C at 1°C intervals), with 1 ng H37Rv template per reaction for IS6110 primer set (A) and IS1081 primer set (B), respectively.
