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. 2019 Sep 13;10:2135. doi: 10.3389/fmicb.2019.02135

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Copyright © 2019 Jiao, Wang, Wang, Wang, Xiao, Sun, Li, Wen, Zhang, Ma, Huang and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimized reaction time for multiplex MCDA assay. Four different duration times (A, 10 min; B, 20 min; C, 30 min; D, 40 min) were determined at optimal amplification temperature (67°C). Biosensors 1, 2, 3, 4, 5, and 6 represent genomic DNA template of H37Rv 10 pg, 1 pg, 100 fg, 10 fg, 1 fg, 100 ag. Biosensors 7 and 8 represent negative control (genomic DNA from M. abscessus 1 ng) and blank control (DW).