TABLE 1.
The sequences of primers used in this study.
| Assay | Primers | Sequence (5′–3′) | Target gene |
| MTB-MCDA | 6110-F1 | CGCGGTCAGCACGATT | IS6110 |
| 6110-F2 | GACGCGGTCTTTAAAATCG | IS6110 | |
| 6110-CP1 | TCTCCGCGCAGCCAACACGGAG TGGGCAGCGAT | IS6110 | |
| 6110-CP2 | CCGGGACCACGACCGAAGACGCA ATTCGGCGTTGTC | IS6110 | |
| 6110-C2 | CCGGGACCACGACCGAAGA | IS6110 | |
| 6110-D2 | GCTGAGCTGAAGCGCTT | IS6110 | |
| 6110-R1 | GCACCCACTTACGCAC | IS6110 | |
| 6110-R2 | AGGCGCAGGTCGATGC | IS6110 | |
| 1081-F1 | TGGCGACCTGCTACCT | IS1081 | |
| 1081-F2 | TGTGCACCCCGACGAC | IS1081 | |
| 1081-CP1 | GGAAAGCTTTGTCACACCAAGTG TGCTGGGAGTATCCACTCG | IS1081 | |
| 1081-CP2 | CTCGATGCCGGCCCGTATTCG CGCACCTTGAGC | IS1081 | |
| 1081-C2 | CTCGATGCCGGCCCGTAT | IS1081 | |
| 1081-D2 | CGCCGACGCCCTGGT | IS1081 | |
| 1081-R1 | ATCGACACTTGCGACTT | IS1081 | |
| 1081-R2 | ATGGCCAAAGAGCTCGA | IS1081 | |
| Primers labeled | 6110-C1∗ | 5′-FITC-TCTCCGCGCAGCCAACA-3′ | IS6110 |
| 6110-D1# | 5′-biotin-AAGTAGACGGGCGACCT-3′ | IS6110 | |
| 1081-C1∗ | 5′-FITC-GGAAAGCTTTGTCACACCAAGTGT-3′ | IS1081 | |
| 1081-D1# | 5′-biotin-GACCAGGCGCTCCATC-3′ | IS1081 |
FITC, fluorescein isothiocyanate.