TABLE 2.
Genomic DNA used to determine the specificity of MCDA.
| Bacteria | Strain no. (source of strains)a | No. of strains | MCDA resultb |
| M. tuberculosis | H37Rv | 1 | P |
| BCG | 1 | P | |
| Isolated strains (BCH) | 20 | P | |
| Klebsiella pneumoniae | Isolated strains (BCH) | 1 | N |
| Streptococcus pneumonia | Isolated strains (BCH) | 1 | N |
| Pseudomonas aeruginosa | Isolated strains (BCH) | 1 | N |
| Listeria monocytogenes | Isolated strains (BCH) | 1 | N |
| Staphylococcus epidermidis | Isolated strains (BCH) | 1 | N |
| Staphylococcus aureus | Isolated strains (BCH) | 1 | N |
| Bacillus cereus | Isolated strains (BCH) | 1 | N |
| Enterobacter sakazakii | Isolated strains (BCH) | 1 | N |
| Campylobacter jejuni | Isolated strains (BCH) | 1 | N |
| Escherichia coli | Isolated strains (BCH) | 1 | N |
| Aeromonas hydrophila | Isolated strains (BCH) | 1 | N |
| Pseudomonas Shigella | Isolated strains (BCH) | 1 | N |
| Salmonella | Isolated strains (BCH) | 1 | N |
| Shigella flexneri | Isolated strains (BCH) | 1 | N |
| Shigella boydii | Isolated strains (BCH) | 1 | N |
| Shigella dysenteriae | Isolated strains (BCH) | 1 | N |
| Shigella sonnei | Isolated strains (BCH) | 1 | N |
| Vibrio parahaemolyticus | Isolated strains (BCH) | 1 | N |
| Vibrio vulnificus | Isolated strains (BCH) | 1 | N |
| Enterococcus faecium | Isolated strains (BCH) | 1 | N |
| Enterococcus faecalis | Isolated strains (BCH) | 1 | N |
| M. fortuitum | Isolated strain (BCH-NTCL) | 1 | N |
| M. abscessus | Isolated strain (BCH-NTCL) | 1 | N |
| M. avium | Isolated strain (BCH-NTCL) | 1 | N |
| M. intracellulare | Isolated strain (BCH-NTCL) | 1 | N |
| M. malmoense | Isolated strain (BCH-NTCL) | 1 | N |
| M. kansasii | Isolated strain (BCH-NTCL) | 1 | N |
| M. gordonae | Isolated strain (BCH-NTCL) | 1 | N |
| M. chelonei | Isolated strain (BCH-NTCL) | 1 | N |
| M. flavescens | Isolated strain (BCH-NTCL) | 1 | N |
aBCH, Beijing Children’s Hospital; BCH-NTCL, National Tuberculosis Clinical Laboratory, Beijing Chest Hospital. bP, positive; N, negative. Only genomic DNA templates from MTBC could be detected by MCDA assay, indicating the extremely high specificity of the method.