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. 2019 Sep 13;10:1157. doi: 10.3389/fphys.2019.01157

FIGURE 6.

FIGURE 6

Western blot analysis of NLRP3 inflammasome components and AQP2 in primary cultured rat IMCD cells incubated with Ang II (1 nM) and co-treated with the NF-κB inhibitor Bay 11-7082 (100 μM) or the AT1 receptor blocker valsartan (100 μM). (A) Semi-quantitative immunoblots probed with anti-p-NF-κB and NF-κB antibodies. (B) The p-NF-κB/NF-κB ratio. (C) Semi-quantitative immunoblots probed with anti -NLRP3, -ACS, -caspase-1, -IL-1β and -β-actin antibodies. (D) Corresponding densitometric analyses of NLRP3, ACS, caspase-1 and IL-1β protein expression corrected for β-actin. (E) Semi-quantitative immunoblots probed with anti-AQP2 and -β-actin antibodies. (F) Corresponding densitometric analyses of AQP2 protein expression corrected for β-actin. NF-κB, transcription factors of the nuclear factor κB; p-NF-κB, phosphorylated-NF-κB (Ser536); NLRP3, Nod-like receptor pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1, an evolutionarily conserved enzyme that proteolytically cleaves other proteins; IL-1β, interleukin-1β; ∗p < 0.05 compared with control group; #p < 0.05 compared with Ang II group.