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. 2019 Sep 19;10:4272. doi: 10.1038/s41467-019-12080-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Design and biophysical properties of BG505 SOSIP-I53-50A.1NT1. a Schematic representation of the computational docking protocol used to identify trimeric NP components suitable for fusion to BG505 SOSIP. r and ω were sampled during docking to minimize d while avoiding clashes. The C termini of BG505 SOSIP and N termini of the I53-50A trimer are shown as red and blue spheres, and the dashed line indicates the linker that connects BG505 SOSIP and I53-50A. b Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of BG505 SOSIP and BG505 SOSIP-I53-50A.1NT1 under reducing (left) and non-reducing (right) conditions. c SEC chromatogram of BG505 SOSIP-I53-50A.1NT1 with peaks corresponding to aggregates and trimers annotated (left) and blue native (BN)-PAGE of trimer fractions (right). d NS-EM analysis of PGT145/SEC-purified BG505 SOSIP-I53-50A.1NT1 with BG505 SOSIP (light blue) and I53-50A.1NT1 (dark blue) annotated. Shown are 2D-class averages. e Glycan profile of BG505 SOSIP-I53-50A.1NT1 as determined by HILIC-UPLC with complex glycans in pink and oligomannose glycans in green. The pie chart represents the percentage of total complex glycans vs. oligomannose glycans per trimer. The percentages of Man_5-9GlcNAc₂ glycans (M5–M9) are listed in the table for BG505 SOSIP and BG505 SOSIP-I53-50A.1NT1. Source data are provided as a Source Data file